Mikologiya I Fitopatologiya 51(2):110–116 Kudryavtzeva NN, Pobedinskaya MA, Kokaeva LY, Zaitchik BT, Kutuzova IA, Azarkovich MI, Elansky SN, Valueva TA (2017) The proteolytic activity and virulence of Alternaria alternata strains, isolated from tomato. Kokaeva LY, Kudryavtzeva NN, Pobedinskaya MA, Zaitchik BT, Elansky SN (2015) Virulence of Alternaria alternata strains isolated from different organs of host plants toward different potato and tomato cultivars. Kapsa JS (2008) Important threats in potato production and integrated pathogen/pest management. Griffith GW, Shaw DS (1998) Polymorphisms in Phytophthora infestans: four mitochondrial haplotypes are detected after PCR amplification of DNA from pure cultures or from host lesions. Gannibal PB, Yli-Mattila T (2007) Morphological and UP-PCR analyses and design of a PCR assay for differentiation of Alternaria infectoria species-group. Wageningen University Lelystad, The Netherland, pp 151–156 doi: 10.1094/Phyto-74-537Įlansky S, Pobedinskaya M, Kokaeva L, Statsyuk N, Alexandrova A (2012) Molecular identification of the species composition of Russian isolates of pathogens, causing early blight of potato and tomato.
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Using the specific primers, it was possible to exclude the Alternaria disease in some leaves with symptoms similar to early blight.Īndersen B, Thrane U (1996) Differentiation of Altemaria infectoria and Alternaria alternata based on morphology, metabolite profiles, and cultural characteristics. Alternaria infectoria occurred in potato and tomato samples occurred at a somewhat lower frequency (13 and 8%, respectively). Alternaria solani was found in 30 and 35% leaves, respectively.
It was detected in 50% of tested potato leaves and 41% of tested tomato leaves (alone or in complex). Alternaria alternata (sensu lato) was the most abundant species. All three Alternaria pathogens were present in blighted leaves alone or in complex ( A. Constructed primers were applied for the survey of early blight agents on affected potato and tomato leaves collected in different regions of Russia. The specificity of primers was confirmed by the absence of amplified products after PCR with DNA of Alternaria spp., other potato and tomato pathogenic fungal species and plant DNA. Three species-specific PCR primer sets were designed for Alternaria alternata sensu lato, Alternaria solani and Alternaria infectoria identification. Alternaria species are the causal agents of potato and tomato early blight disease.
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